To investigate the therapeutic effect of the herpes simplex virus I-thymidine kinase gene (HSV1-tk)/ganciclovir (GCV) system on human ovarian cancer.
The nude mice tumors were formed by injection of human ovarian cancer cell line AO and AO/HSV1-tkc (AO cells carried with HSV1-tk gene which was constructed in China) subcutaneously, and then were transplanted to the omentum of nude mice. The filter-passing culture fluid of VPC/HSV1-tkc was injected daily into the peritoneum of the nude mice in the group of in vivo. Finally, all of the three groups of nude mice (control, in vivo, ex vivo) accepted the treatment of GCV.
The inhibitory rate of the RV/HSV1-tkc/GCV to AO tumors were 46.8%, and, only could the residual microscopic tumors be seen in most of the nude mice omentum transplanted with AO/HSV1-tkc tumor after GCV treatment.
GCV could more effectively inhibit the growth of the human ovarian tumors carried with HSV1-tk gene which was transplanted onto the nude mice omentum than which transplanted subcutaneously; The application of the HSV1-tk/GCV system would be very promising in the gene therapy of ovarian cancer.
Download Full PDF Version (Non-Commercial Use)